Positive or negative electrical charges are frequently related to biomolecules. Once placed in an electrical field, charged biomolecules move towards the conductor of opposite charge thanks to the development of static attraction. Natural action is that the separation of charged molecules in associate degree applied force field. The relative quality of individual molecules depends on many factors. The foremost vital of that area unit web charge, charge/mass quantitative relation, molecular form and therefore the temperature, body and body of the matrix through that the molecule migrates. Complicated mixtures will be separated to terribly high resolution by this method. Hydrated gel networks have several fascinating properties for electrophoresis. They permit a good style of automatically stable experimental formats like horizontal/vertical electrophoresis in block gels or electrophoresis in tubes or capillaries. The mechanical stability conjointly facilitates post action manipulation